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Do not attempt to do anything you have not been trained to do by ePSIC s= taff.
Wob= ble objective lens and set CL3 to minimise change in illumination area. Thi= s ensures parallel illumination.
Insert a condenser lens aperture of 20um or smaller (CLA2, 4) an= d centre.
Never swap to diffraction mode without a small condenser lens aperture i= nserted
Ensure that area of interest is illuminated and insert a selecte= d area aperture if required.
Ensure phosphor screen is lowered.
Press =E2=80=98Diff=E2=80=99 on microscope panel.
Select the required camera length on TEMCentre.
Using the projector lens deflectors move the central spot to the= beam stop position (marked on the phosphor screen).
Using the small focus screen, focus the diffraction pattern with= diff focus knob.
Exposing the small focus screen to the central beam for prolonged period= s may damage the screen.
If necessary correct for intermediate lens astigmatism with the = IL stig deflectors.
Insert the OneView camera and lift the phosphor screen. <= /p>
Using the = projector lens deflectors mover the central spot so that it is entirely cov= ered by the beam stop.
Exposing the OneView cam= era to the central beam for prolonged periods may damage the camera.
Record your diffraction pattern.
Don=E2=80=99t leave the diffraction pattern on the OneView camer= a for longer than is necessary.
Press =E2=80=98Mag=E2=80=99 on the microscope panel to return to= imaging mode.