- At the start of the day perform a high flash and run up the emission.
- Open the beam valve and check there is a beam on the large phosphor screen. Centre with beam shift.
- Insert and centre the largest condenser lens aperture (150μm)
- Ensure the objective lens is at standard focus and bring the sample to Gaussian focus height.
- Select the alpha value which you want to use.
- Select the spot size you with to use.
- Adjust any 2-fold astigmatism in the beam using condenser lens stig.
- Anode wobble and adjust beam deflectors (GunA2) until concentric movement is achieved.
- Go to 1 Mx magnification, find a feature on the sample and wobble the HT. Correct for any movement in the sample with beam align deflectors (CLA2).
- Correct for tilt balance using the tilt wobbler.
- Iterate steps 7-10 if necessary.
- At 1 Mx magnification illuminate a region of amorphous material, spread the beam, insert the One-View camera and lift the viewing screen.
- View a live fft FFT of the image and adjust two fold astigmatism in the objective lensusing the corrector optics panel (make sure the toggle button is on TEM).
- If necessary run the COSMO corrector.
- Insert the required condenser lens aperture for imaging.