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  1. At the start of the day perform a high flash and run up the emission.
  2. Open the beam valve and check there is a beam on the large phosphor screen. Centre with beam shift.
  3. Insert and centre the largest condenser lens aperture (150μm)
  4. Ensure the objective lens is at standard focus and bring the sample to Gaussian focus height.
  5. Select the alpha value which you want to use.
  6. Select the spot size you with to use.
  7. Adjust any 2-fold astigmatism in the beam using condenser lens stig.
  8. Anode wobble and adjust beam deflectors (GunA2) until concentric movement is achieved.
  9. Go to 1 Mx magnification, find a feature on the sample and wobble the HT. Correct for any movement in the sample with beam align deflectors (CLA2).
  10. Correct for tilt balance using the tilt wobbler.
  11. Iterate steps 7-10 if necessary.
  12. At 1 Mx magnification illuminate a region of amorphous material, spread the beam, insert the One-View camera and lift the viewing screen.
  13. View a live fft of the image and adjust two fold astigmatism in the objective lens.
  14. If necessary run the COSMO corrector.
  15. Insert the required condenser lens aperture for imaging.

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