For Samples smaller than the FOV:

Method 1: Calculating the shift

1. GTS Rotation 0°

2. Draw annotation circle same size as sample/feature of interest.

3. Configure to be at Y 1280 and X 1080.

4. Move sample/feature using X step to recover it in the centre of circle.

5. GTS Rotation 90

6. Move sample/feature using Z step to recover it in the centre of the circle.

7. Check positions at 180 and 270 and with full rotation (0-360) to ensure feature remains centered.

Method 2: Circle method (Easier, slightly less precise)

1. GTS Rotation 0°

2. Draw annotation circle same size as sample/feature of interest.

3. Configure to be at Y 1280 and X 1080.

4. Move sample/feature using X step to recover it in the centre of circle.

5. GTS Rotation 90

6. Move sample/feature using Z step to recover it in the centre of the circle.

7. Check positions at 180 and 270 and with full rotation (0-360) to ensure feature remains centred.