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š Centering your sample
Ā For Samples smaller than the FOV:
Method 1: Calculating the shift
GTSĀ Rotation 0Ā°
Draw annotation circle same size as sample/feature of interest.
Configure to be at Y 1280 and X 1080.
Move sample/feature using X step to recover it in the centre of circle.
GTS Rotation 90
Move sample/feature using Z step to recover it in the centre of the circle.
Check positions at 180 and 270 and with full rotation (0-360) to ensure feature remains centered.
Method 2: Circle methodĀ (Easier, slightly less precise)
GTSĀ Rotation 0Ā°
Draw annotation circle same size as sample/feature of interest.
Configure to be at Y 1280 and X 1080.
Move sample/feature using X step to recover it in the centre of circle.
GTS Rotation 90
Move sample/feature using Z step to recover it in the centre of the circle.
Check positions at 180 and 270 and with full rotation (0-360) to ensure feature remains centred.
Ā For Samples larger than the FOV:
For Samples larger than the FOV:
Mount sample on GTS
Set GTS Rotation to 0 degrees
Use GTS X to move the specimen until one side aligns with the edge of the FOV.
Record the GTS X position
Use GTS X to move the sample in the opposite direction until the other edge aligns with the FOV.
Record the 2nd GTS X Position.
Calculate the centre of these two points and enter the value into GTS X.
Set GTS Rotation to 90 degrees.
Repeat steps 3 to 7 using GTS Z motion.
Once sample is centred along X and Z axis the sample will be on the COR.
Optimising your imaging quality
No of projections is Pixel dimensions x Ļ/2
Flat field ideally should be 35K count, contrast between brightest and darkest feature in sample should be around 20%
For stitching multiple tomography scans leave at least 10-20% overlap.
Image Guided Diffraction
Ā Using reconstructed images to guide diffraction
Diffraction tomography